For a video format to support Alpha Channels you need at least 8 bits per channel and four (4) channels for a total of 32-bits. Compressed formats are 8 bit color per channel for a with a total of 24 bits. Quicktime JPEG 2000, Cineware - free from GoPro, and several other formats support alpha channels. Best for graphics without color complexity or gradients.
GIF (Graphic Interchange Format): Index file with the potential for transparency.
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PDF supports massive amounts of transparency depending on the version. PDF (Portable Document Format): PDFs are a staple for Print and online documents. It can be both Raster and Vector and be used for Print and Web. SVG (Scalable Vector Format): SVG files are a modern day sensation. This format is best for graphics and videos with high complexity in color. PNG (Portable Network Graphics File): PNG was made for transparency on web, however, it is used quite a bit in video as well. Of course there are other formats, but these mezzanine or intermediate/smart codecs are some of the best for ongoing editing. MOV/J2K): For Offline/Online Video Editing MOV/Animation): For Offline/Online Video Editing MOV/PNG): For Offline/Online Video Editing MOV/Prores 4444): For Offline/Online Video Editing
MOV/DNxHD/HR): For Offline/Online Video Editing Quicktime (.MOV/Cineform): For Offline/Online Video Editing.If you want transparency, for web or editorial, here are your best options: CSS, Flash and HTML 5 Video which have been used to trick transparency for MP4 files have been used before. Just as a side note, there are very few acceptable video files with transparency for web. Exporting your files into this format will be completely flattened. MP4 in it's current variation and format cannot have transparency at all.
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